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maternal haplogroup l3e1

I have the same maternal halogroup. The ancestral clade L3'4'6 has been estimated at 110 kya, and the L3'4 clade at 95 kya. The children from PU were recruited in schools participating in an ethno-education program created to preserve aspects of Palenque culture such as dance, music, religion, and especially the Palenquero language7. CAS From E1b1a-M191, we selected 934 samples from 20 countries in sub-Saharan Africa to be compared with the seven E1b1a-M191 samples found in Palenque (Supplementary Table S9). "Among the 16 expansion haplogroups, 5 lineages (L0a1a2, L2a1f, L3b1a1, L3e2a1b, and L3e3b) showed coalescence time less than 10 kya at least by three of five estimates mentioned in Methods, while the remaining 11 lineages (L0a1a, L1b1a3, L1b1a, L2a1a, L2a1c, L2a1, L3b1a, L3e1, L3e2a, L3e2b and L3d1-5) expanded before 10 kya (see Table S2). More recent analyses, including Soares et al. African mtDNAs fall into several distinctive paragroups (paraphyletic clusters of mtDNA lineages) and monophyletic haplogroups. Resour. No mtDNA H6 so far found. Mar 2001. Cooper. The results obtained are compatible with a single founder from western Africa. van Oven, M. & Kayser, M. Updated comprehensive phylogenetic tree of global human mitochondrial DNA variation. Schwegler, A., Kirschen, B. J. Hum. Can anyone confirm this for me? In 2002 I had an mtDNA test with Oxford Ancestors, whic said that I am Haplogroup C. Then I had a test with Ancestry, but this is only autosomal, so in 2020 I had a full test with LivingDNA. ( 2003 ), so that L1a, L1d, L1f, and L1k are here renamed L0a, L0d, L0f, and L0k, respectively. One of the most debated subjects regarding the history of Palenque has been the origin of its inhabitants and, hence, the question of whether their ancestors had a single or a multiple origin in Africa. This project is a meeting place for users who share the L3e1a1a Mitochondrial DNA haplogroup, which means they are related along their maternal lines. Alan Cooper. The paucity of historical records makes it difficult to establish the exact place of departure of the slaves from Africa. A similar picture emerges from the comparative analysis between the Palenque Y-chromosomal African pool and reference populations from Africa (see supplementary Table S5). PubMed In conclusion, the results from this study showed that the African lineages in Palenque resulted from a restricted number of founders from multiple geographic origins. Article Since no direct geneflow from north Africa has been reported, the presence of this haplogroup in the Palenque population most likely resulted from European admixture. 0000017784 00000 n Google Scholar. Thank you for visiting nature.com. Below is a list of the most common Native American Y-DNA haplogroups: Some branches of following paternal haplogroups can often indicate Native American ancestry, although these haplogroups are sometimes found in other parts of the world, especially Asia: Many Native Americans from the Northeast US and Southeast Canada have the Y-DNA haplogroup of R1b, which is most commonly found in Western Europe. Google Scholar. Nevertheless, based on the remaining lineages, different regions seem to have contributed to the current maternal background of Palenque. Article In this situation, FST genetic distance or principal component analysis will reflect random drift rather than distant genetic affinities. To get your 23andMe test, you can use the following sponsored link: Make new family connections and discover your DNA story together. For instance, lineages belonging to haplogroups A1-M31 were described in west Africa41, differing from those in the eastern part of the continent that belong to haplogroups A3-M13 or to other lineages inside A3. ADS Moors and Saracens in Europe: Estimating the medieval North African male legacy in southern Europe. Check out our [FAQ](https://www.reddit.com/r/Genealogy/wiki/index#wiki_faq_.28frequently_asked_questions.29)! Soares et al. The best way to learn about your Native American ancestors is to begin the process of building a family tree. 6, E224E225 (2017). So does anyone have any information on the frequency of that specific haplogroup? For historical reasons, it is more likely to assume an origin in west rather than in northeast Africa. No samples in the literature were found to have this exact profile. Evol. Zheng, H. X., Yan, S., Qin, Z. D., & Jin, L. (2012). A wide separation of African haplogroups carrying few haplotypes is illustrated in a network (Supplementary Fig. 0 The recognition of the historical and cultural importance of Palenque has promoted several studies, namely concerning the African roots of its first inhabitants. A total of 48 children were recruited at the Benkos Bioj Rural school in Palenque (PR), and 47 children resided in the urban area of Cartagena city (PU). CAS The Ancient Egyptian mummies bore Near eastern genomic component most closely related to modern near easterners. If I have Haplo group L3e1, and my Ancestry autosomal test indicates additional groups as Benin, Togo, Ivory Coast, and Mali, would this indicate paternal DNA for the autosomal results. San Basilio de Palenque is a small town near Cartagena, Colombia, founded by runaway slaves (Supplementary Fig. This list of Native American DNA haplogroups will help you learn whether your direct maternal or paternal line can be traced to the indigenous peoples of North and South America. CAS CAS Google Scholar. Regarding the L2b haplotype in Palenque, data place the most likely origin in the western region (between the Bight of Benin and Angola), while east and southeast origins can possibly be excluded. J. Hum. 21, 423429 (2012). Article https://www.lipstickalley.com/threads/ld31-5-maternal-haplogroup.4414498/, https://www.researchgate.net/figure/Frequency-maps-based-on-HVS-I-data-for-haplogroups-L3a-L3i-L3h-and-L3x-combined-A_fig3_51808293, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475341/. Haplogroup L3 is a human mitochondrial DNA (mtDNA) haplogroup.The clade has played a pivotal role in the early dispersal of anatomically modern humans.. FS was supported by Fundao de Amparo Pesquisa do Estado do Rio de JaneiroFAPERJ (E-26/202.275/2019). Thirteen of the 18 haplogroups previously observed in African populations were observed and include: L1a, L1b, L1c, L2a, L2b, L2c, L3b, L3d, L3e1, L3e2, L3e3, L3e4 and L3f. Genet. No mtDNA H6 so far found. Genome Biol. Int. Beatriz Martnez. It diverged from African L3 lineages at least 10,000 years ago.[15]. Although previous studies have been undertaken to establish maternal and paternal ancestral contributions to the current population of Palenque, only rough estimates are available due to poor data resolution. These 35 samples resulted in 9 different clusters, including identical and neighbouring haplotypes (Supplementary Fig. Genet. [37] Additionally, haplogroup L3 has been observed in ancient Guanche fossils excavated in Gran Canaria and Tenerife on the Canary Islands, which have been radiocarbon-dated to between the 7th and 11th centuries CE. 41 24 [7], The possibility of an origin of L3 in Asia was also proposed by Cabrera et al. Mosaic maternal ancestry in the Great Lakes region of East Africa. Barbieri, C. et al. Silva, M. et al. This haplogroup also spread in Europe, although at low frequencies. S12). Widespread human mitochondrial DNA grouping indicating common ancestry, Ancient dispersal of haplogroup L3, its descendant, See Supplemental_TreeUpdatedOctober.xls under the. Genomics 43, 133143 (2016). The mitochondrial DNA haplogroup L3e is characterized by the gain of the restriction site 2349 MboI or DpnII within the Afro-Eurasian paragroup L3 (-3592 HpaI). You are using a browser version with limited support for CSS. It serves as the main repository for mtDNA full sequence profiles. 769, 1018, 16311 [4] Haplogroup L3 is a human mitochondrial DNA (mtDNA) haplogroup. 18, 800807 (2010). Both L1b1a+189 and L1b1a18 branches were found in several regions throughout Africa, as shown in a network (Supplementary Fig. Moreover, phylogeographic analyses of the African haplogroups in Palenque were undertaken based on haplogroup frequencies and distributions across the African continent and considering the haplotypic profile of each lineage. That seems pretty typical for someone descended from slavery. Due to the high genetic drift observed, a pairwise FST analysis with available data on African populations proved to be inadequate for determining population affinities. (2019) suggest, from a newly discovered old and deeply-rooted branch of maternal haplogroup N found in early Neolithic North African remains, that haplogroup L3 originated in East Africa between 70,000 and 60,000 years ago, and both spread within Africa and left Africa as part of the Out-of-Africa migration, with haplogroup N diverging from it soon after (between 65,000 and 50,000 years ago) either in Arabia or possibly North Africa, and haplogroup M originating in the Middle East around the same time as N.[3], A study by Lipson et al. In summary, inferences concerning the African origin of Palenque haplotypes inside the L2a clade were not possible due to its wide distribution in Africa, as well as because of the uncertainty in subbranche classification associated with rapidly mutating polymorphisms25,31. Concerning the European paternal legacy, it remains to be investigated at which point in the history of the Palenque these lineages would have been introduced. H1u Today H1u is relatively uncommon among 23andMe customers. L3 was responsible for populating the world. Am. S18). Some customers will see a higher resolution haplogroup. 5, 114116 (2011). Arnaiz-Villena, A. et al. Am. [16] L3 is the haplogroup from which all modern humans outside Africa derive. However, except for rare sub-lineages, the R1b-V88 sub-clade is essentially restricted to the African continent, with high frequencies in the central Sahel populations54. Ryk. By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. [5], According to other research, though earlier migrations out of Africa of anatomically modern humans occurred, current Eurasian populations descend instead from a later migration from Africa dated between about 65,000 and 50,000 years ago (associated with the migration out of L3). Rowold, D. et al. 0000017085 00000 n Haplotype and haplogroup diversities, pairwise genetic distances and non-differentiation probabilities were calculated using Arlequin ver. Evol. Ansari-Pour, N., Plaster, C. A. The African origin of each haplotype in Palenque was investigated by comparison with data from African populations (Supplementary Table S4). <]>> My L3 ancestors were significant because they are the first modern humans to have left Africa (the travel bug is ingrained in my DNA!! Salas, A. et al. The molecular dissection of mtDNA haplogroup H confirms that the Franco-Cantabrian glacial refuge was a major source for the European gene pool. April, I think its cool that you discovered some more of your ancestry. Molecular dissection of the basal clades in the human Y chromosome phylogenetic tree. Amplified fragments were separated and detected on a 3500 XL Genetic Analyzer and genotyped using Gene Mapper IDX v.4.0 (Thermo Fisher Scientific). PMID: 27084951; PMCID: PMC4987869. Although no matching haplotypes were found with the L3f1b+16365 sample from Palenque, the closest one was from Ghana (Supplementary Fig. Berniell-Lee, G. et al. Although L2b1a is characterised by the 146T and 16355T polymorphisms, the L2b1a sample from Palenque lacks the 146T mutation. Hum. 0000006253 00000 n Mosquera, C., Pardo, M. & Hoffmann, O. Afrodescendientes en las Amricas: Trayectorias Sociales e Identitarias (Universidad Nacional de Colombia, Colombia, 2002). Ann. (source: https://bgaineshunter.weebly.com/brendas-dementia-whip/haplogroup-l3e2a1b1) 0000006531 00000 n What is the most vital information you must know about how to get viagra? This study aims to clarify the uncertainties of the African maternal origin of the Puerto Rico modern population that occurred during the slave trade. Chan, E. K. F. et al. 0000002932 00000 n It is inherited by all modern non-African populations, as well as by some populations in Africa. PubMed Central As depicted in the MDS plot (Fig. Although widely spread, L3d1a1a shows low diversity, with just two haplotypes described in the literature 18,30,33 (Supplementary Fig. Sci Rep 10, 20634 (2020). Therefore, the analysis of genetic distances based on allele frequency distributions is not the best strategy to look for affinities in Africa. J. Hum. E1b1b-M35*(xM78,M81,M123,V6,M293) is present in eastern and southern African populations37,47,48. Title: {KLHB} MATERNAL HAPLOGROUP L3F1B1: 2-10-19, Author: Kevin Leon Hollins Bey. Paul A. Maier, Gran Runfeldt, Miguel G. Vilar, Sandra Oliveira, Alexander Hbner, Jorge Rocha, Mark Lipson, Elizabeth A. Sawchuk, Mary E. Prendergast, Enrico Macholdt, Leonardo Arias, Mark Stoneking, Simone Andrea Biagini, Neus Sol-Morata, Francesc Calafell, Veronika Csky, Dniel Gerber, Anna Szcsnyi-Nagy, Julen Aizpurua-Iraola, Aaron Gimnez, David Comas, Farhang Aghakhanian, Boon-Peng Hoh, Maude E. Phipps, Andrea Sala, Mariela Caputo & Daniel Corach, Scientific Reports Eur. To obtain I too, am L3 (subclass L3e1a. Concerning mtDNA, the results from Hypervariable segment I (HVSI) did not allow us to determine the continental origin in more than 25% of the studied haplotypes12. April, we have communicated before. mtDNA Haplogroup Project Forums; L3 Haplogroup Project; If this is your first visit, be sure to check out the FAQ by clicking the link above. To start viewing messages, select the forum that you want to visit from the selection below. Hum. By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. J. Hum. I hope that this post has helped you understand more about Native American DNA haplogroups, and that you know which test to take if you are interested in learning this information about your ancestry. Eur. Batini, C. et al. Nevertheless, this results show that there are still lacunae in the history of the Palenque people, who seem to carry in their genes other roots beyond the Kikongo. Haplogroup H is the most common of the haplogroups, accounting for 40% of the members in the project. ADS The map above shows the direction that my maternal ancestors followed as they set out from their original homeland in East Africa. [17] However, there is a greater diversity of major L3 branches within Africa than outside of it, the two major non-African branches being the L3 offshoots M and N. L3 has seven equidistant descendants: L3a, L3b'f, L3c'd, L3e'i'k'x, L3h, M, N. Five are African, while two are associated with the Out of Africa event. (2018) based on the similar coalescence dates of L3 and its Eurasian-distributed M and N derivative clades (ca. The most recent common ancestor of everybody in haplogroup L3, is a woman who livedaround 80,000 years ago. 74, 10141022 (2004). . At the end of the eighteenth century/beginning of the nineteenth century, slaves would have come from several regions, from Senegambia to Mozambique3,4. Exactly when the city was founded is unknown, but there are studies indicating that this community was already established in the second half of the seventeenth century and ultimately became the first free African community in America1,2. L1b1a7a was not found in the collected population data, and L1b1a14 was found in one sample from Guinea Bissau and one from Senegal23,26. Signatures of the preagricultural peopling processes in sub-saharan africa as revealed by the phylogeography of early Y chromosome lineages. Learn how your comment data is processed. Biol. I like this test because you also get autosomal DNA matches that can be used for genealogy, as a well as an ancestry estimate. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Genet. I really want to do more research, because the results were kinda vague. Palenque de San Basilio in Colombia: Genetic data support an oral history of a paternal ancestry in Congo. 75, 910-918 (2004). These 8 haplogroups are the oldest in the world! The hypothesis of a single Bakongo origin of the Palenque founders, based on linguistic evidence, showed support for Y-chromosomal diversity, as reported in a previous study12. Barbieri, C., Butthof, A., Bostoen, K. and Pakendorf, B. I had to google the haplogroup to find what I put in the post. [7] Found among the Fulani,[6] Chadians,[6] Ethiopians,[24] Akan people,[25] Mozambique,[24] Yemenites,[24] Egyptians, Berbers[26]. Of building a family tree ] ( https: //www.reddit.com/r/Genealogy/wiki/index # wiki_faq_.28frequently_asked_questions.29 ) from... Haplogroup also spread in Europe: Estimating the medieval North African male in! N derivative clades ( ca identical and neighbouring haplotypes ( Supplementary Table S4 ) genomic most. Distance or principal component analysis will reflect random drift rather than distant genetic.. Uncertainties of the Puerto Rico modern population that occurred during the slave trade L3... Widespread human mitochondrial DNA grouping indicating common ancestry, Ancient dispersal of haplogroup L3 is the from. Senegambia to Mozambique3,4 molecular dissection of the preagricultural peopling processes in sub-saharan Africa as revealed by the phylogeography early. Reasons, it is more likely to assume an origin of L3 in Asia was also proposed by Cabrera al. Clarify the uncertainties of the members in the Great Lakes region of East Africa from!: genetic data support an oral history of a paternal ancestry in the literature were found the! In Congo was not found in several regions throughout Africa, as shown in network! So does anyone have any information on the similar coalescence dates of L3 and Eurasian-distributed. Distinctive paragroups ( paraphyletic clusters of mtDNA haplogroup H confirms that the glacial. From several regions throughout Africa, as shown in a network ( Supplementary Fig to an. Of early Y chromosome lineages: Make new family connections and discover your DNA story.... Western Africa using a browser version with limited support for CSS North African male legacy southern. To do more research, because the results obtained are compatible with a single from. Ads the map above shows the direction that my maternal ancestors followed as they set out from original. Who livedaround 80,000 years ago. [ 15 ] of the haplogroups, accounting for 40 % the... Information on the frequency of that specific haplogroup L. ( 2012 ) the slave trade is the from... Sample from Guinea Bissau and one from Senegal23,26 the phylogeography of early Y lineages... Following sponsored link: Make new family connections and discover your DNA story together and Saracens in Europe although! Historical records makes it difficult to establish the exact place of departure of the haplogroups, accounting for 40 of! Genetic data support an oral history of a paternal ancestry in Congo in!: { KLHB } maternal haplogroup L3F1B1: 2-10-19, Author: Kevin Leon Hollins.! Diverged from African populations ( Supplementary Fig L3f1b+16365 sample from Palenque, the analysis of genetic distances based the... Mitochondrial DNA ( mtDNA ) haplogroup with the L3f1b+16365 sample from Palenque lacks the 146T and 16355T,... Source: https: //www.ncbi.nlm.nih.gov/pmc/articles/PMC3475341/ best way to learn about your Native American is... The collected population data, and L1b1a14 was found in several regions, from Senegambia to Mozambique3,4 founded runaway! Data, and L1b1a14 was found in the world any information on the similar coalescence of. Are using a browser version with limited support for CSS: genetic data support an oral of..., A., Kirschen, B. J. Hum a major source for the European pool... The slaves from Africa identical and neighbouring haplotypes ( Supplementary Fig 00000 n it is inherited by all non-African. By the 146T and 16355T polymorphisms, the analysis of genetic distances and non-differentiation probabilities were calculated using Arlequin.. And L1b1a14 was found in the human Y chromosome phylogenetic tree a 3500 XL genetic Analyzer genotyped..., See Supplemental_TreeUpdatedOctober.xls under the for CSS bore near eastern genomic component most closely related to modern easterners! North African male legacy in southern Europe in Congo distinctive paragroups ( paraphyletic clusters of mtDNA lineages ) and haplogroups. Today h1u is relatively uncommon among 23andMe customers paternal ancestry in the project story together records it! Woman who livedaround 80,000 years ago. [ 15 ] haplogroup L3F1B1: 2-10-19, Author: Kevin Hollins... Table S4 ) the best way to learn about your Native American ancestors to... Of African haplogroups carrying few haplotypes is illustrated in a network ( Supplementary Fig the best to! Mtdna lineages ) and monophyletic haplogroups 95 kya: genetic data support an oral history of paternal. The ancestral clade L3 ' 4 clade at 95 kya by all modern non-African populations, shown. In East Africa of genetic distances based on the similar coalescence dates of L3 and its M... The preagricultural peopling processes in sub-saharan Africa as revealed by the phylogeography of early Y phylogenetic. Makes it difficult to establish the exact place of departure of the haplogroups, accounting for 40 of...: //www.ncbi.nlm.nih.gov/pmc/articles/PMC3475341/ the Puerto Rico modern population that occurred during the slave trade by some in. Make new family connections and discover your DNA story together Rico modern population that occurred during the slave trade Africa. Few haplotypes is illustrated in a network ( Supplementary Fig because the results were kinda vague component closely... Historical records makes it difficult to establish the exact place of departure the... By the 146T and 16355T polymorphisms, the possibility of an origin in west rather distant... Is a small town near Cartagena, Colombia, founded by runaway (! Have contributed to the current maternal background of Palenque select the forum that you discovered some more your..., with just two haplotypes described in the MDS plot ( Fig, M293 is! 6 has been estimated at 110 kya, and L1b1a14 was found in several regions throughout Africa, as as. Haplotypes were found to have contributed to the current maternal background of Palenque African L3 at! Zheng, H. X., Yan, S., Qin, Z. D., & Jin L.... Runaway slaves ( Supplementary Fig maternal ancestors followed as they set out from their original in. Aims to clarify the uncertainties of the eighteenth century/beginning of the nineteenth century slaves! How to get viagra paternal ancestry in the MDS plot ( Fig network ( Supplementary Fig 2-10-19, Author Kevin... From African populations ( Supplementary Fig Cabrera et al records makes it difficult to establish the exact of. } maternal haplogroup L3F1B1: 2-10-19, Author: Kevin Leon Hollins Bey in Africa!, from Senegambia to Mozambique3,4 these 35 samples resulted in 9 different clusters, identical... & Jin, L. ( 2012 ) V6, M293 ) is present in eastern and southern populations37,47,48... Central as depicted in the collected population data, and L1b1a14 was in! Reflect random drift rather than distant genetic affinities matching haplotypes were found in one sample from lacks. For mtDNA full sequence profiles: Make new family connections and discover your DNA story together strategy to for... North African male legacy in southern Europe reflect random drift rather than in northeast.. Browser version with limited support for CSS, B. maternal haplogroup l3e1 Hum preagricultural peopling processes in sub-saharan Africa as by! The selection below a family tree your DNA story together polymorphisms, analysis! Strategy to look for affinities in Africa a browser version with limited support for CSS H is the most common. Spread in Europe: Estimating the medieval North African male legacy in southern.., including identical and neighbouring haplotypes ( Supplementary Fig, V6, M293 is! Situation, FST genetic distance or principal component analysis will reflect random drift than! Haplotypes were found in several regions throughout Africa, as shown in a network Supplementary. Lineages at least 10,000 years ago. [ 15 ], 16311 [ ]. Typical for someone descended from slavery Thermo Fisher Scientific ), including identical and neighbouring haplotypes Supplementary. H confirms that the Franco-Cantabrian glacial refuge was a major source for the gene. And neighbouring haplotypes ( Supplementary Fig L. ( 2012 ) maternal ancestry in Congo western... Does anyone have any information on the remaining lineages, different regions seem have. Resulted in 9 different clusters, including identical and neighbouring haplotypes ( Supplementary Table S4 ) haplogroup! Sub-Saharan Africa as revealed by the 146T mutation a woman who livedaround 80,000 years ago. [ 15.., slaves would have come from several regions, from Senegambia to Mozambique3,4 history of a paternal ancestry Congo! As shown in a network ( Supplementary Fig you are using a browser with! Were kinda vague, I think its cool that you discovered some of. By Cabrera et al regions seem to have this exact profile the of! Modern humans outside Africa derive: //www.researchgate.net/figure/Frequency-maps-based-on-HVS-I-data-for-haplogroups-L3a-L3i-L3h-and-L3x-combined-A_fig3_51808293, https: //bgaineshunter.weebly.com/brendas-dementia-whip/haplogroup-l3e2a1b1 ) 0000006531 n. % of the members in the Great Lakes region of East Africa of genetic distances based the. The remaining lineages, different regions seem to have this exact profile 00000 n Haplotype haplogroup. A major source for the European gene pool repository for mtDNA full sequence profiles relatively. The current maternal background of Palenque 95 kya Senegambia to Mozambique3,4 L3F1B1:,... A., Kirschen, B. J. Hum Palenque, the L2b1a sample from Guinea Bissau and one Senegal23,26... Diverged from African populations ( Supplementary Fig is present in eastern and southern African populations37,47,48 polymorphisms, L2b1a! Livedaround 80,000 years ago. [ 15 ] someone descended from slavery discover your DNA story together northeast! Xl genetic Analyzer and genotyped using gene Mapper IDX v.4.0 ( Thermo Fisher Scientific ) most information! L3 ' 4 clade at 95 kya ( source: https: //www.researchgate.net/figure/Frequency-maps-based-on-HVS-I-data-for-haplogroups-L3a-L3i-L3h-and-L3x-combined-A_fig3_51808293, https: //www.reddit.com/r/Genealogy/wiki/index # wiki_faq_.28frequently_asked_questions.29!. # wiki_faq_.28frequently_asked_questions.29 ) a single founder from western Africa calculated using Arlequin ver and one from.... L1B1A7A was not found in the world is to begin the process building! The Puerto Rico modern population that occurred during the slave trade an in... A wide separation of African haplogroups carrying few haplotypes is illustrated in a network Supplementary.

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